[PDF] Lentivirus Gene Engineering Protocols Methods In Molecular Biology Ebook
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Addgene: pCas34 Plasmid pCas34 from Dr. Brian Pfleger's lab contains the insert gRNA and is published in Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 10.1016/j.ymben.2016.07.007. This plasmid is available through Addgene. Transfection - Wikipedia Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells.In animal cells, transfection is the preferred term as ... Addgene: lentiGuide-Puro Plasmid lentiGuide-Puro from Dr. Feng Zhang's lab contains the inserts S. pyogenes sgRNA cassette and Puromycin resistance and is published in Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. This plasmid is available through Addgene.
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